INTERNATIONAL TRAINING COURSE ON THE PRINCIPLES AND PROTOCOLS OF MYCOBACTERIUM TUBERCULOSIS GENOTYPING.

COURSE TITLE: GENOTYPE ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS ISOLATES

COURSE ORGANISER:

Philip Nelson Institute of Medical Research.

RESOURCE PERSON:   Philip  Ifesinachi  Anochie

                                     Research Scientist/ ISID/ESCMID Fellow.

E-MAIL: philipanochie@yahoo.co.uk.

             philipanochie@gmail.com

COURSE CONTENT: The course contains 17 modules.

1. Competent preparation and storage of the reagents for the diagnosis for the presence of Mycobacterium strains in sputa and other clinical specimens by light microscopy, culture and isolation of Mycobacterium strains from sputum and other specimens using essentially the Loweinstein-Jensen (LJ) medium, characterization and identification of isolated strains by both cultural , biochemical and molecular methods like Hain assay, spoligotyping, MIRU etc, establishment of the sensitivity profiles of the isolates to various anti-TB drugs using LJ slopes ,the proportion method and the BACTEC method.

2· GenoQuick ®MTB: Rapid molecular genetic assay for the direct detection of the Mycobacterium tuberculosis complex from patient specimens.

3· GenoType ® MTBC: Molecular genetic assay for the differentiation of the Mycobacterium tuberculosis complex from cultured material.

4· GenoType ®Mycobacterium CM: Molecular genetic assay for the identification of the clinically most relevant mycobacterial species from cultured material.

5· GenoType® MTBDRplus: Molecular genetic assay for the identification of resistance to rifampicin and/or isoniazid of the Mycobacterium tuberculosis complex.

6· GenoType® MTBDRsl: Molecular genetic assay for the identification of resistances to fluoroquinolones, aminoglycosides/ cyclic peptides, and ethambutol of the Mycobacterium tuberculosis complex.

7· Spoligotyping: A PCR-based method to simultaneously detect and type Mycobacterium tuberculosis complex bacteria for epidemiological studies.

8· BACTEC TM MGIT TM 960 SIRE test for the antimycobacterial susceptibility testing of Mycobacterium tuberculosis for first and second line drugs.

9· Capilia TB-Neo for the detection of Mycobacterium tuberculosis complex from patient specimens.

10· PCR and reverse hybridization assays.

11· DNA inactivation and extraction from positive MGIT tubes.

12· DNA inactivation and extraction from positive LJ tubes.

13· DNA inactivation and extraction from sediments.

14· Chemical DNA extraction.

15· MIRU-VNTR : Mycobacterial interspersed repetitive units variable number tandem plus repeats; a strain identification and differentiation protocol for the epidemiological study of Mycobacterium tuberculosis.

16· GT-BLOT 20 automated equipment for reverse hybridization assays.

17. dsRNA genome extraction, gel electrophoresis and RT-PCR of dsRNA.

TEACHING METHODS AND MATERIALS: The course has been created by experts in online training. It is delivered via a web browser and requires a computer with an internet connection. All the reading material is embedded in an electronic format within the online course. There are no formal assessment criteria for this course, however, each of the modules contains a final test which will be used to assess your knowledge of each of the topics.

APPLICATION: The course is available to any laboratory personnel who wishes to gain a working knowledge of tuberculosis genotyping techniques and to assess how to learn the importance of tuberculosis genotyping on their practice, though it is generally aimed at tuberculosis laboratory personnel and Physicians at Chest Clinics and DOTS Centers.

As the course is self-directed, motivated individuals who are able to manage their own time and learning effectively, will gain the greatest benefit from the course.

You can apply for this course at any time and you will be given 3 months to complete the course. An extension can be applied for, if required. A Professional Proficiency  Certificate in Mycobacterium tuberculosis genotyping will be issued to successful participants.

Contact E-mail: philipanochie@yahoo.co.uk  for further information and details.

 

The  Training  is powered by : Philip Nelson Institute of Medical Research.

                                                      E-mail: philipnelsoninstitute@yahoo.com.

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